Concentration calculator

Reconstitution math for NAD+, expressed as weight/volume percent (1% w/v equals 10 mg/mL).

What this calculates

Weight/volume percent describes how much solid is dissolved in a given volume of liquid: a 1% w/v solution is one gram of material in 100 mL, which is the same thing as 10 mg/mL. The calculator multiplies your target percentage by your final volume to give the mass of NAD+ that volume requires.

Worked through: a 2% w/v solution at 30 mL needs 2% of 30 mL expressed as mass — 0.6 g, or 600 mg. Halve the volume and you halve the mass; the concentration is unchanged. This is arithmetic about a solution, not a recommendation about a quantity.

What to check before preparing NAD+

• Starting material. PROVENANCE FIRST. PubChem's physical-description field for CID 5892 reads "Highly hygroscopic white solid; [Merck Index] White powder; [Sigma-Aldrich MSDS]". Those are a reference work and a supplier safety data sheet, not a published characterisation study and not a determination by any regulator. There is no approved product label and no United States Pharmacopeia monograph for this substance to check it against. source
• Solubility. No solubility figure is published for NAD+ on this site, because none was found in a primary source. Without one there is no way to say from the published record whether a given target concentration is achievable.
• Storage. Stability is the specific thing FDA faulted, and it did so twice. In its September 5, 2019 proposed rule evaluating NAD as a bulk drug substance, FDA wrote that NAD "is well characterized physically and chemically, but it degrades substantially when exposed to light, moisture, alkaline pH, or standard room temperatures and would not be stable under ordinary storage conditions absent multiple compensatory measures". FDA recorded the same finding separately for the reduced form, NADH. No numeric shelf life, no in-use period and no reconstituted-solution condition is established by any source located during data entry: there is no pharmacopoeial monograph and no approved product label for this substance, so no body with standing to determine handling conditions has done so. Any storage figure circulating for this compound traces to a supplier page rather than to a determination by anyone. source

What the number does not tell you

The result assumes the vial contains the mass its label states and that the material is what the label says it is. Neither is established by arithmetic. Both come from an assay — what HPLC and mass spectrometry establish — and the published record on NAD+ has limits worth reading before treating any figure as settled.